In dogs, Otitis infection is one of the common problem associated with the ears and infection is flared up with a number of bacterial and yeast organisms. The most commonly isolated bacteria from the ear canals of dogs affected by otitis are Staphylococcus spp. Along with Staphylococcus , other microorganisms commonly associated with otitis include Pseudomonas, Proteus, Enterococcus, Streptococcus and Malassezia spp. All these lead to significant discomfort and pruritus. In the present study, a total of 31 samples from otitis infection were collected and examined for the presence of different microbes present in the infection. Different microbes comprising of both bacteria and yeast were found. Predominantly Staphylococcus sps., Pseudomonas sps, Proteus sps were noticed and minorly Streptococcus, and few yeasts like Malassezia and Candida albicans. In one sample multiple number of Pastuerella sps in groups .along with Pseudomonas and Staphylococcus were noticed. On cultural and biochemical tests, it was confirmed as Pastuerella multocida. P. multocida is considered as a highly versatile gram-negative coccobacillus and is frequently harboured with the normal oropharyngeal microbiota of livestock, with the highest rate of presence among cats (70–90%) and dogs (20–55%). It was indeed an abnormal finding of the presence of Pastuerella multocida in multiple number and is clearly an indication of sub clinical meningitis and may lead to

meningoencephalomyelitis.